Epidermal Culture Media
CELLnTEC has the following specialised epidermal keratinocyte media available for 2D keratinocyte culture:
| Cat # | Description | Species | PCT | Defined | Price |
|---|---|---|---|---|---|
| CnT-57 | PCT Epidermal Keratinocyte Medium, Low BPE | Human | $99.60 | ||
| CnT-57CF | PCT Epidermal Keratinocyte Medium, Low BPE, Ca free | Human | $105.00 | ||
| CnT-07 | PCT Epidermal Keratinocyte Medium | Human & Mouse | $99.60 | ||
| CnT-02 | Epidermal Keratinocyte Medium | Human & Mouse | $99.60 | ||
| CnT-03 | PCT Epidermal Keratinocyte Medium | Rat | $112.00 | ||
| CnT-33 | Epidermal Keratinocyte Medium | Rat | $122.00 | ||
| CnT-09 | Epidermal Keratinocyte Medium | Canine | $112.00 | ||
| All media supplied as kits containing both 500mL basal medium and separate frozen supplements | |||||
3D Keratinocyte Culture:
Please visit our Epidermal 3D Page.
CnT-57
For the absolute best isolation and growth, high colony forming efficiency and extended longevity, CnT-57 combines all the progenitor cell retention benefits of a PCT medium with the isolation and growth boost of BPE.
An additional benefit of CnT-57 is its low-BPE formulation. Traditional epithelial culture media typically use high BPE concentrations (e.g. Invitrogen and Cambrex media falling in the range of 25-50 ug/mL). In contrast, the optimum concentration of BPE in the PCT CnT-57 has been found to be 6 ug/mL. Thus this medium provides all the benefits of BPE whilst minimizing any potential variability and the influence of unwanted factors.
As a result of the PCT formulation, differentiation of cells in PCT media may be delayed. For any experiments in which cells are induced to differentiate, cultures should be switched to the non-PCT equivalent medium 24-hours prior to the induction of differentiation by calcium switch or equivalent (see below for details).
This medium has a recommended seeding density and protocol for differentiation - see below.
Special formulations are also possible, for example hydrocortisone-free, and others. Please contact us with your specific needs.
CnT-07
When a fully defined environment is required, the Progenitor Cell Targeted (PCT) medium CnT-07 provides best possible isolation efficiency and growth rate, in the absence of serum or BPE, via maximized progenitor cell retention.
As a result of the PCT formulation, differentiation of cells in PCT media may be delayed. For any experiments in which cells are induced to differentiate, cultures should be switched to the non-PCT equivalent medium 24-hours prior to the induction of differentiation by calcium switch or equivalent (see below for details).
This medium has a recommended seeding density and protocol for differentiation - see below.
Special formulations are also possible, for example hydrocortisone-free, and others. Please contact us with your specific needs.
CnT-02
CnT-02 is based on a highly optimized basal formulation and supplement profile, but does not include the novel PCT supplements. Whilst it will support growth of human and mouse keratinocytes as traditional defined media do, we recommend its use primarily during differentiation experiments.
This medium has a recommended seeding density - see below.
Special formulations are also possible, for example hydrocortisone-free, and others. Please contact us with your specific needs.
Important Note on 2D Differentiation: CnT-07 and CnT-57 are recommended for isolation and maintenance of keratinocyte progenitor cells (human and mouse). However for 2D differentiation of these cells, we recommend the use of CnT-02, because the PCT formulation of CnT-07 and CnT-57 can delay differentiation.
As with all differentiation work, it is important that cells reach confluency before calcium levels are increased. In addition, to the addition of calcium, cells should also be switched to CnT-02, a non-PCT medium. For our recommended differentiation protocol, please click here.
One of the important considerations is seeding density. For our fully-defined media, we recommend the following seeding densities when starting out:
- Human epidermal keratinocytes: 4x10^4 cells per sq. cm. at isolation, 4x10^3 per sq. cm. when passaging
- Mouse epidermal keratinocytes: 4x10^4 cells per sq. cm. at isolation, 2x10^4 per sq. cm. when passaging
Accordingly when starting with small tissue samples, it may be necessary to reduce the initial size of your culture vessels to obtain optimal seeding density and growth rates. Once you have experienced the growth of your cells, densities should be adjusted to obtain the desired passaging frequency.
