Human Exosomes

ZenBio human pre-adipocytes (adipose derived adult stem cells) and placental mesenchymal stem cells (MSC) are characterized by their self-renewing capacity and ability to differentiate into chondrocytes, adipocytes, and osteocytes. This makes them attractive starting materials for tissue engineering and regenerative medicine applications.

Since few transplanted cells persist in vivo, the beneficial effects of cell therapy may lie in the secreted factors being the active component of this treatment. A key part of paracrine secretion is Exosomes, which are membrane vesicles that are stored intracellularly in endosomal compartments and are secreted when these structures fuse with the cell plasma membrane.

• Exosomes contain protein, DNA, and RNA, thus making them an attractive vector of paracrine signals delivered by stem cells.
• Exosomes may also be "loaded" with predetermined proteins and nucleic acid to achieve a desired effect (1).
• Exosomes can be stored as an "off-the-shelf" product having the potential for circumventing many of the limitations of viable cells for therapeutic applications in regenerative medicine.
• In vitro, exosomes from pre-adipocytes stimulate cell proliferation in a wound healing model.
• In vivo, adipose-graft derived exosomes have been shown to be a promising tool for skin repair and remodeling (2)

Quality Control: All cells have been screened negative for HIV-1, HIV-2, Hep-B, Hep-C and Syphilis. All isolated and concentrated exosomes undergo includes Particle mean diameter, Protein concentration, RNA concentration and Concentration of particles/ml.

Exosome-mediated fibroblast proliferation

After the cell monolayer was 'wounded' using a cell scraper, the cultures were washed and fed with DMEM + 10% exosome-free FBS (Control), or DMEM + 10% exosome-free FBS supplemented with 25µg of pre-adipocyte exosomes (Exosome Treated). After a 5 day incubation, fibroblast proliferation closed the gap between the two edges of the wounded monolayer only in the medium supplemented with exosomes. These data support the notion that factors including exosomes secreted by pre-adipocytes (adipose tissue-derived MSC) play a role in wound healing and tissue regeneration, by facilitating cellular proliferation

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